 |
|
 |
 |
AffiSpin® Kits |
Benefits |
- Quick and easy separation of microgram amounts of glycoproteins
- Ready-to-use kit
- Easy handling
- High recovery
- Operation in common centrifuges
- Desalting devices already included
|
 |
 |
 |
Description
AffiSpin-Kits are designed especially for the purification of microgram amounts of glycoproteins. They are ready-to-use and thus provide easy handling. They combine AffiSpin micro columns with the corresponding optimized adsorption and elution buffers and collection tubes. The Kits are designed for operation in a centrifuge, vacuum manifold or with the provided syringes. They facilitate glycoprotein separations in a simple way for fast and reproducible results.
AffiSpin columns are packed with 0.1 mL adsorbent.
The Kit is designed for two affinity separations.
|
 |
 |
 |
 |
 |
Fig: Cap and plug of an AffiSpin column are removed. AffiSpin columns are placed into an open 2 mL collection tube. A sample is dissolved in the corresponding adsorption buffer A and added to the column. |
 |
Fig: The AffiSpin column with a collection tube is placed into a centrifuge. Separation is performed by spinning the open column for 2 min at 500 U/min. |
 |
Application 308:
|
 |
Binding capacity of AffiSpin ConA for the glycoprotein ovalbumin
|
Column: |
AffiSpin ConA column (Cat. No. 051041); 0.1 mL adsorbent |
Mobile Phase A: |
ConA adsorption buffer |
Mobile Phase B: |
ConA elution buffer B2 |
Detection: |
Bradford protein assay |
Sample: |
Ovalbumin; 400 µg dissolved in mobile phase A |
|
 |
The table below shows the results of the lectin affinity separation of the glycoprotein ovalbumin on AffiSpin ConA. 400 µg ovalbumin were added to the AffiSpin ConA column. Flow through, elution and washing steps were performed as described in the protocol for using AffiSpin lectin columns and the fractions were collected and pooled. 187 µg of ovalbumin were bound by the column and could be specifically eluted and recovered. |
 |
|
Total protein applied |
Flow through |
Elution and washing |
Total recovery |
Amount |
400 µg |
161 µg |
187 µg |
348 µg |
Recovery |
|
40% |
47% |
87% |
|
 |
Application 311:
|
 |
Separation of the glycoproteins ovalbumin and ovomucoid on AffiSpin ConA
|
Column: |
AffiSpin ConA (Cat. No. 051041) |
Sample: |
Ovalbumin; 200 µg dissolved in A Ovomucoid; 200 µg dissolved in A |
Mobile Phase A: |
ConA adsorption buffer |
Mobile Phase B: |
ConA elution buffer B2 |
Detection: |
SDS-PAGE |
|
 |
The picture stated below shows the results of the separation of a mixture of two glycoproteins with AffiSpin ConA. 200 µg ovalbumin and 200 µg ovomucoid dissolved in adsorption buffer were mixed and added to the used AffiSpin ConA column. The column was treated as described in the protocol for using AffiSpin lectin columns. Afterwards the samples were analyzed with SDS-PAGE. The gel was stained with Coomassie stain. |
 |
 |
M 3 4 5
6 7 8 |
 |
Legend
Molecular weight marker
Ovalbumin
Ovomucoid
Mixture of ovalbumin and ovomucoid applied to the spin column
Flow through
Elution fraction
Washing step |
|
 |
back to top |
 |
|
Order Information
Product |
Content |
Cat. No. |
Price [€] |
Info |
MSDS |
AffiSpin-WGA Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL WGA Adsorption Buffer
20 mL WGA Elution Buffer B1 |
051031 |
209 |
 |
German
English |
AffiSpin-ConA Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL ConA Adsorption Buffer
20 mL ConA Elution Buffer B2 |
051041 |
179 |
German
English |
AffiSpin-LCH Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL LCH Adsorption Buffer
20 mL LCH Elution Buffer B1 |
051051 |
209 |
German
English |
AffiSpin-PNA Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL PNA Adsorption Buffer
20 mL PNA Elution Buffer B1 |
051061 |
209 |
German
English |
AffiSpin-AIL Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL AIL Adsorption Buffer
20 mL AIL Elution Buffer B1 |
051071 |
209 |
German
English |
AffiSpin-VVL Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL VVL Adsorption Buffer
5 mL VVL Elution Buffer B1 |
051081 |
209 |
German
English |
AffiSpin-AAL Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL AAL Adsorption Buffer
10 mL AAL Elution Buffer B1 |
051091 |
209 |
German
English |
AffiSpin-SNA Kit |
2 spin columns,syringes, adapters and ultra filtration devices
20 ml SNA Adsorption Buffer,
10 ml SNA Elution Buffer B1,
2.5 ml SNA Elution Buffer B2 |
051121 |
229 |
German
English |
AffiSpin-MAL Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL MAL Adsorption Buffer
20 mL MAL Elution Buffer B1 |
051131 |
209 |
German
English |
AffiSpin-UEA Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL UEA Adsorption Buffer
10 mL UEA Elution Buffer B1 |
051141 |
209 |
German
English |
AffiSpin-GNA Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL GNA Adsorption Buffer
10 mL GNA Elution Buffer B1 |
051151 |
209 |
German
English |
AffiSpin-ECL Kit |
2 Spin columns, syringes, adapters and ultra filtration devices
20 mL ECL Adsorption Buffer
20 mL ECL Elution Buffer B1 |
051161 |
209 |
German
English |
|
 |
Pricelist |
 |
back to top |
 |
|
 |
|