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Lectin Affinity Separation |
Lectin Affinity Technology
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Lectin affinity separation is based on the ability of lectins to bind specifically to certain oligosaccharide structures on glycoconjugates. Different lectins display a variety of binding specificities. Lectins have been extensively used to purify glycoproteins, glycopeptides and oligosaccharides from all kinds of biological sources.
This technique is highly selective and thus provides a high purity target compound whilst the biological activity and natural glycosylation pattern is conserved due to the mild separation parameters.
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| Fig: GNA (Galanthus nivalis agglutinin),
complex with 12 molecules of methylmannose
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Process of Lectin Affinity Separation
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- A sample is applied that contains a target glycoprotein being complementary to the binding site of the immobilized lectin
- The target glycoprotein specifically binds to the lectin
- Unbound compounds and contaminants are washed out
- The target glycoprotein is desorbed with a special elution
buffer; the purified and concentrated product can be collected.
The affinity matrix can be regenerated for further use.
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