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GlycoCleave® – Immobilized Enzymes for Glycoanalysis |
| Benefits |
- Particle technique facilitates a quick isolation of your asialo-product
- Enzyme is reusable
- Ready-to-use kits including buffers and reaction cartridges
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Description
The product is a suspension of immobilized neuraminidase (sialidase, N-acetylneuraminyl hydrolase, E.C. 3.2.1.18, from Vibrio cholerae culture filtrates). The enzyme particles (polymer particles, 65 µm) are stored in sodium acetate buffer pH 5.5 containing calcium chloride and sodium chloride.
1 U (unit) liberates 1 µmol N-acetylneuraminic acid from NANA-Lactose incubated for 1 min at +37 ºC and pH 5.5 in sodium acetate buffer. The immobilized neuraminidase has an activity of 1 U/mL settled particles.
Ready-to-use GlycoCleave kits combine the immobilized neuraminidase with reaction cartridges and optimized buffers.
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Areas of application
Vibrio cholerae neuraminidase (E.C. 3.2.1.18) hydrolyses o-ketoside α2-3, α2-4, α2-6 and α2-8 bonds of terminal N-acetylneuraminic acid in oligosaccharides, glycoproteins, gangliosides and polysaccharides. This enzyme can be used for various investigations as for example structural characterization of glycoproteins, as a receptor-destroying enzyme in virology or to characterize and design the binding behavior of glycoconjugates. |
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Legend |
| 1 |
pI-Marker |
| 2 |
native bovine fetuin |
3 +
4 |
fetuin after GlycoCleave neuraminidase treatment |
| 5 |
asialofetuin (acid hydrolysis) |
| A |
Bovine carbonic anhydrase, pl=6.0 |
| B |
β-Lactoglobulin B, pl=5.1 |
| C |
Phycocyanin (3 bands),
pl=4.45, 4.65, 4.75 |
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| Isoelectric focussing of bovine fetuin before and after treatment with GlycoCleave neuraminidase. In lane 3 und 4 the shift of the pI due to the removal of terminal sialic acids can be seen for the processed glycoprotein. Enzymatic reaction was carried out at 37 ºC for 20 hours. |
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Applications
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Order Information
| Product |
Content |
Amount |
Cat. No. |
Price [€] |
Info |
MSDS |
| GlycoCleave Neuraminidase |
Neuraminidase (vibrio cholerae) immobilized onto polymer particles
1 unit/mL fixed bed |
1.0 mL
2.0 mL |
132011
132012 |
249
399 |
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 German
 English |
GlycoCleave Neuraminidase
Kit I |
Neuraminidase (vibrio cholerae) immobilized makes 600 µL settled particles,
1 unit/mL
reaction tubes, buffer |
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132100 |
200 |
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German
English |
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 Pricelist |
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For customized products
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Protein Purification and
Application: Affinity separation of asialofetuin standard on AffiSep-RCA column