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AffiSep® Lectin Columns and Kits |
| Benefits |
- Applicable with common HPLC and FPLC equipment
- All advantages of high-quality documentation, automation and detection
- Fast separation
- High reproducibility
- Ready-to-use kits
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Description
AffiSep lectin chromatography columns are ready-to-use and can be operated with common HLPC or FPLC equipment. The column back pressure at a flow rate of 1 mL/min is for most columns less than 5 bar. The PEEKTM body of the column is inert and ideal for biochromatography.
The AffiSep lectin column dimensions range from a screening column of only 0.8 mL volume (4.6 x 50 mm) over ideal analytical columns of 1.6 and 2.5 mL up to 4 mL columns for semi preparative isolation of glycoproteins. The adsorbent particles have a dimension of 75 µm and pore diameters of 100 nm. The polymer-based adsorbents provide many advantages compared to conventional agarose based gels, as there is a very good separation performance and much better chemical, mechanical and microbiological stability.
AffiSep Kits combine a column with optimized buffers and column control standards. The monitoring of the column performance can easily be carried out using the provided standards. Standards and buffers can both be purchased separately.
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Applications
Exemplary Capacity data

| Product |
Column Volume |
Human serum applied |
Dynamic Capacity: bound and eluted protein [µg] |
| AffiSep-ConAColumn |
0,8 mL |
75 µL |
915 |
| AffiSep-LCH Column |
0,8 mL |
75 µL |
780 |
| AffiSep-GNA Column |
0,8 mL |
75 µL |
120 |
| AffiSep-AIL Column |
0,8 mL |
75 µL |
315 |
| AffiSep-WGA Column |
0,8 mL |
75 µL |
340 |
| AffiSep-SNA Column |
0,8 mL |
75 µL |
480 |
| AffiSep-MAL Column |
0,8 mL |
75 µL |
230 |
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Order Information
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| Product |
Volume |
Dimension |
Cat. No. |
Price [€] |
Info |
MSDS |
| AffiSep-WGA Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011031 011032 011033 011034 |
269 319 399 519 |
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German
English |
| AffiSep-ConA Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011041 011042 011043 011044 |
209 239 279 319 |
German
English |
| AffiSep-LCH Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011051 011052 011053 011054 |
269 319 399 519 |
German
English |
| AffiSep-PNA Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011061 011062 011063 011064 |
269 319 399 519 |
German
English |
| AffiSep-AIL Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011071 011072 011073 011074 |
269 319 399 519 |
German
English |
| AffiSep-VVL Column |
1.6 mL |
4.6 x 100 mm |
011082 |
499 |
German
English |
| AffiSep-AAL Column |
0.8 mL 1.6 mL 2.5 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm |
011091 011092 011093 |
399 599 999 |
German
English |
| AffiSep-SNA Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011121 011122 011123 011124 |
329 409 499 799 |
German
English |
| AffiSep-MAL Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011131 011132 011133 011134 |
269 319 399 519 |
German
English |
| AffiSep-UEA Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011141 011142 011143 011144 |
329 409 499 799 |
German
English |
| AffiSep-GNA Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011151 011152 011153 011154 |
269 319 399 519 |
German
English |
| AffiSep-ECL Column |
0.8 mL 1.6 mL 2.5 mL 4.0 mL |
4.6 x 50 mm 4.6 x 100 mm 4.6 x 150 mm 4.6 x 250 mm |
011161 011162 011163 011164 |
329 409 499 799 |
German
English |
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| Product |
Content |
Cat. No. |
Price [€] |
Info |
MSDS |
| AffiSep-WGA Kit |
AffiSep-WGA column 0.8 mL
WGA Adsorption buffer, 1000 mL
WGA Elution buffer B1, 100 mL
GP-Standard Fetuin, 5 mg
Car-Standard PNP-α-D-Glc; PNP-β-D-GlcNAc, 1 mL |
031031 |
369 |
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German
English |
| AffiSep-ConA Kit |
AffiSep-ConA column 0.8 mL
ConA Adsorption buffer, 1000 mL
ConA Elution buffer B2, 100 mL
GP-Standard ovalbumin, 5 mg |
031041 |
309 |
German
English |
| AffiSep-LCH Kit |
AffiSep-LCH column 0.8 mL
LCH Adsorption buffer, 1000 mL
LCH Elution buffer B1, 100 mL
GP-Standard Glucose Oxidase, 5 mg |
031051 |
369 |
German
English |
| AffiSep-PNA Kit |
AffiSep-PNA column 0.8 mL
PNA Adsorption buffer, 1000 mL
PNA Elution buffer B1, 100 mL
GP-Standard Asialofetuin, 5 mg |
031061 |
369 |
German
English |
| AffiSep-AIL Kit |
AffiSep-AIL column 0.8 mL
AIL Adsorption buffer, 1000 mL
AIL Elution buffer B2, 100 mL
GP-Standard Asialofetuin, 5 mg
Car-Standard PNP-β-D-Lac; PNP-α-D-Gal, 1 mL |
031071 |
369 |
German
English |
| AffiSep-VVL Kit |
AffiSep-VVL column 1.6 mL
VVL Adsorption buffer, 1000 mL
VVL Elution buffer B2, 50 mL
Car-Standard PNP-α-D-Glc; PNP-α-D-GalNAc, 1 mL |
031082 |
599 |
German
English |
| AffiSep-AAL Kit |
AffiSep-AAL column 0.8 mL
AAL Adsorption buffer, 1000 mL
AAL Elution buffer B1, 100 mL
Car-Standard PNP-β-L-Fuc; PNP-β-D-GlcNAc, 1 mL |
031091 |
499 |
German
English |
| AffiSep-SNA Kit |
AffiSep-SNA column 0.8 mL
SNA Adsorption buffer, 1000 mL
SNA Elution buffer B1, 100 mL
SNA Elution buffer B2, 5 mL
GP-Standard Fetuin, 5 mg |
031121 |
429 |
German
English |
| AffiSep-MAL Kit |
AffiSep-MAL column 0.8 mL
MAL Adsorption buffer, 1000 mL
MAL Elution buffer B, 100 mL
GP-Standard Fetuin, 5 mg |
031131 |
369 |
German
English |
| AffiSep-UEA Kit |
AffiSep-UEA column 0.8 mL
UEA Adsorption buffer, 1000 mL
UEA Elution buffer B, 100 mL
Car-Standard PNP-α-D-Glc; PNP-β-L-Fuc, 1 mL |
031141 |
429 |
German
English |
| AffiSep-GNA Kit |
AffiSep-GNA column 0.8 mL
GNA Adsorption buffer, 1000 mL
GNA Elution buffer B, 100 mL
GP-Standard Glucose oxidase, 5 mg |
031151 |
369 |
German
English |
| AffiSep-ECL Kit |
AffiSep-ECL column 0.8 mL
ECL Adsorption buffer, 1000 mL
ECL Elution buffer B1, 100 mL
GP-Standard Asialofetuin, 5 mg |
031161 |
429 |
German
English |
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Pricelist |
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