 Description
GlycoImage® Lectin Array Kit is designed especially for the screening of glycconjugates, e.g. antibodies. The microplate surface presents a panel of lectins. These lectins provide a broad range of specificities.
The glycosylated sample binds to the lectin surface of the microplate. The sample-lectin-affinity depends on the glycan structure of the sample. A higher affinity causes higher signal intensity.
Enzymatic detection is possible after biotinylation of the sample or by using a sample-specific, biotinylated antibody. The biotinylation reagents are included to the kit. |
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The results show glycan profiles of the determined samples. The samples presented in the charts above are glycoproteins. Chart 1 shows the glycan profile of bovine fetuin, a sialylated protein. Chart 2 presents the glycan profile of bovine fetuin after neuraminidase treatment. The glycan profile (chart 2) shows an increase of signal intensity on galactose-binding lectins on the one hand, on the other hand there is a decrease in signal intensity on sialic-acid specific lectins.
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| Kit Contents |
- 2x modified 96well or 1x 384well microplate
- glycoprotein conjugate (reference)
- streptavidin enzyme conjugate
- substrate (dry substance)
- GlycoImage® Assay Buffer
- GlycoImage® Assay Equilibration Buffer
- GlycoImage® Assay Detection Buffer
- Instruction booklet
- Biotin-NHS
- Biotin solvent
- Labeling buffer
- Dialysis buffer
see specifications for more details
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| Specifications |
| Microplate: |
96well (12x8well stripes), transparent
Alternatively 384well, clear
It is required to use at least multichannel pipets or a washer if handling 384well plates. |
| Glycoprotein Conjugate: |
biotinylated Fetuin |
| Substrate: |
p-Nitrophenylphosphate |
| Wavelength: |
405 nm |
| Sample Volume: |
50µL /well (96well), 20µL/well (384well) |
| Sample type: |
Glycosylated molecules (peptides, proteins, antibodies and -fragments, cells) |
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Protein Purification and
Kits for Glycan Analysis
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