Sialic Acid Kits - Determination of conjugated sialic acid in milk and milk-derived samples

Description GlycoImage® Sialic Acid Kits are designed for the determination of conjugated, bioactive sialic acid in milk and milk-derived samples. The results are expressed as "SA = sialic acid activity". The analytical kit allows a quick and direct comparison of up to 15 samples. Optimized protocols for whey and caseinomacropeptide are provided. Acid oligosaccharides and sialylated glycoconjugates show prebiotic effects. Many other physiological effects are described in the literature [1]. Due to these effects the compounds may derive important ingredients in dietary products, e.g. infant food, whey products and functional food. Unfortunately bound sialic acid shows a limited stability in view of conventional manufacturing steps like heating, drying or protein hydrolysis. The degradation of bound sialic acid results in a decrease of its biological (e.g. prebiotic) activity. This can be monitored using the value of SA = sialic acid activity. The Sialic acid activity needs to be preserved for products of higher value! The analysis using the GlycoImage® Sialic Acid Kit provides a fast and easy activity control of your products, raw materials or other samples of interest. Preservation of the sialic acid activity may require a modification of manufacturing steps, the choice of high value raw materials or improvement of storage conditions of the ingredients and products. [1] Wang, B.; Brand-Miller, J.; McVeagh, P.; Petocz, P.; Concentration and distribution of sialic acid in human milk and infant formulas; Am J Clin Nutr 2001; 74, pp. 510-515

Applications

Kit Contents
modified 96well/ 384well microplate lectin conjugate enzyme conjugate substrate (dry substance) Assay Buffer Assay Equilibration Buffer Assay Detection Buffer Instruction booklet including an analysis assistant sheet (.xls) see specifications for more details
Specifications
Microplate:	96well (12x8well stripes), clear Alternatively 384well, clear It is required to use at least multichannel pipets or a washer if handling 384well plates.
Lectin Conjugate:	biotinylated lectin from Sambucus nigra ( α2,6-bound sialic acid) or biotinylated lectin from Maackia amurensis ( α2,3-bound sialic acid)
Substrate:	p-Nitrophenylphosphate
Wavelength:	405 nm
Sample Volume:	50µL /well (96well), 20µL/well (384well)
Sample type:	whey, caseinomacropeptide (or other milk-derived samples)

GlycoImage® Sialic Acid Kits*

GlycoImage^® Sialic Acid Kits*