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Buffers (for Affinity chromatography) |
| Benefits |
- Optimized buffers for adsorption and elution
- Adsorption buffers contain all contents for stabilizing lectin structure and activity
- Elution buffers contain complimentary carbohydrates for a mild and specific desorption of the bound glycoconjugate
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Description
Adsorption buffers are carefully selected and tested for each lectin. Elution buffers are optimized for defined applications and recommended for each lectin. In some cases different elution buffers are available for one lectin. The carbohydrate concentration can be different for high and low affinity interactions or even different sugars are available for an improved elution specificity and efficiency.
The more complimentary the elution sugar is to the lectin binding site the higher is the affinity towards it and the concentration for desorption of the glycoproteins can be reduced. |
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Application
Lectin affinity separation of α1-glycoprotein on AffiSep-SNA column. The two chromatograms show elution profiles of α1-glycoprotein using different buffer systems. Elution buffer B2 contains sialyllactose which has higher affinity for SNA than lactose (Elution buffer B1). Thus elution can be achieved in a smaller volume and with a lower sugar concentration using sialyllactose.
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Order Information
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| Product |
Content |
Amount |
Cat. No. |
Price [€] |
Info |
MSDS |
Adsorption Buffer
Bis-Tris pH 6.0 |
for lectins ConA, LCH, GNA, ECL, VVL, WGA, MAL, UEA, AAL |
1000 mL |
091180 |
89 |
 |
 German
 English |
Adsorption Buffer
HEPES pH 8.0 |
for lectins PNA, AIL, SNA |
1000 mL |
091181 |
89 |
German
English |
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| Adsorption buffers are delivered as dry substance to be reconstituted with aqua bidest. |
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| Product |
Content |
Amount |
Cat. No. |
Price [€] |
Info |
MSDS |
| WGA Elution Buffer B1 |
N-Acetyl-Glucosamine |
500 mL |
101033 |
89 |
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German
English |
| WGA Elution Buffer B2 |
N,N'-Diacetylchitobiose |
5 mL
10 mL |
101034
101035 |
198
345 |
German
English |
| ConA Elution Buffer B1 |
Mannose |
500 mL |
101043 |
149 |
German
English |
| ConA Elution Buffer B2 |
Methyl-Mannose |
500 mL |
101046 |
89 |
German
English |
| LCH Elution Buffer B1 |
Methyl-Mannose |
500 mL |
101053 |
89 |
German
English |
| PNA Elution Buffer B1 |
Galactose |
500 mL |
101063 |
89 |
German
English |
| PNA Elution Buffer B2 |
Methyl-Galactose |
250 mL
500 mL |
101065
101066 |
89
149 |
German
English |
| AIL Elution Buffer B2 |
Methyl-Galactose |
100 mL
250 mL |
101075
101076 |
89
149 |
German
English |
| VVL Elution Buffer B2 |
N-Acetyl-Galactosamine |
50 mL |
101086 |
349 |
German
English |
| AAL Elution Buffer B1 |
L-Fucose |
100 mL |
101092 |
89 |
German
English |
| SNA Elution Buffer B1 |
Lactose |
500 mL |
101123 |
89 |
German
English |
| SNA Elution Buffer B2 |
Sialyl-Lactose |
5 mL
10 mL |
101161
101162 |
169
345 |
German
English |
| MAL Elution Buffer B1 |
Lactose |
500 mL |
101133 |
89 |
German
English |
| UEA Elution Buffer B1 |
L-Fucose |
100 mL |
101142 |
89 |
German
English |
| GNA Elution Buffer B1 |
Methyl-Mannose |
500 mL |
101153 |
89 |
German
English |
| ECL Elution Buffer B1 |
Methyl-Galactose |
100 mL
250 mL |
101182
101183 |
89
149 |
German
English |
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| Most elution buffers are delivered as dry substance to be reconstituted with aqua bidest. |
 Pricelist |
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Protein Purification and
Reagents
Application: Elution of bound α1-glycoprotein from AffiSep-SNA with lactose