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Protein Quantification |
| SDS PAGE (Sodium dodecylsulfate polyacrylamide gel-electrophoresis) |
· analysis of protein mass and purity
· staining techniques: silver staining, coomassie staining (only microgram amount of protein needed)
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| IEF (Isoelectric focussing) |
· analysis of the isoelectric point of a protein pI
· proteins are separated in an electric field through a pH-gradient located in the gel
Protein and Peptide characterization using HPLC-MS/MS
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 BioPharma |
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